Literature summary for 4.3.1.3 extracted from

Baedeker, M.; Schulz, G.E.
Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase (2002), Structure, 10, 61-67.

Search for more literature for 4.3.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of C273A/D145A, C273A/F329A and C273A/F329G double mutants at 2.25 A, 2.0 A and 1.9 A resolution, respectively	Pseudomonas putida
crystal structure of native histidase inactivated with L-cystein at 1.0 A and Y280F mutant histidase at 1.7 A	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
A142D	3.4% of wild-type activity	Pseudomonas putida
A142G	93% of wild-type activity	Pseudomonas putida
C273A/D145A	no activity, crystal structure	Pseudomonas putida
C273A/F329A	0.04% of wild-type activity, crystal structure	Pseudomonas putida
C273A/F329G	no activity, crystal structure	Pseudomonas putida
D145A	no activity	Pseudomonas putida
F329A	0.04% of wild-type activity	Pseudomonas putida
F329G	no activity	Pseudomonas putida
G141A	1.1% of wild-type activity	Pseudomonas putida
G144A	37% of wild-type activity	Pseudomonas putida
Y280F	0.9% of wild-type activity	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
L-cysteine	10 mM, complete inactivation at pH higher than 10 in the presence of O2	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-histidine	Pseudomonas putida	-	urocanate + NH3	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	P21310	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant histidase	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-histidine	-	Pseudomonas putida	urocanate + NH3	-	r

Synonyms

Synonyms	Comment	Organism
HAL	-	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)