Literature summary for 4.3.1.3 extracted from

Khanna, R.; Chang, T.M.S.
Characterization of L-histidine ammonia-lyase immobilized by microencapsulation in artificial cells: preparation, kinetics, stability, and in vitro depletion of histidine (1990), Int. J. Artif. Organs, 13, 189-195.

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Application

Application	Comment	Organism
synthesis	enzyme can be successfully microcapsulated within cellulose nitrate artificial cells	Pseudomonas fluorescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
20	-	L-histidine	enzyme in solution or microencapsulated	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas fluorescens

Storage Stability

Storage Stability	Organism
37°C, enzyme in solution: 50% loss of activity after 9.5 days, microencapsulated enzyme: 50% loss of activity after 15 days	Pseudomonas fluorescens
4°C, enzyme in solution: 27% loss of activity after 21 days, and 60% loss of activity after 77 days, microencapsulated enzyme: 5% loss of activity after 21 days, and less than 50% loss of activity after 77 days	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-histidine	natural substrate	Pseudomonas fluorescens	urocanate + NH3	-	?

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Release 2023.1 (January 2023)