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Literature summary for 4.3.1.3 extracted from

  • Klee, C.B.; Kirk, K.L.; Cohen, L.A.; McPhie, P.
    Histidine ammonia-lyase. The use of 4-fluorohistidine in identification of the rate-determining step (1975), J. Biol. Chem., 250, 5033-5040.
    View publication on PubMed
Search for more literature for 4.3.1.3

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-L-histidine competitive Pseudomonas sp.
L-N-gamma-methylhistidine weak competitive Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.
1.25
-
 4-fluoro-DL-histidine in the presence of 0.1 mM CdCl2 Pseudomonas sp.
2.7
-
 DL-histidine in the presence of 0.1 mM CdCl2 Pseudomonas sp.
3
-
 Urocanate
-
 Pseudomonas sp.
5
-
 4-fluorourocanate
-
 Pseudomonas sp.
6.4
-
 4-fluoro-DL-histidine in the presence of 1 mM EDTA Pseudomonas sp.
10
-
 DL-histidine in the presence of 1 mM EDTA Pseudomonas sp.
20
-
 2-fluorohistidine
-
 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 ATCC 11,299b
-

Reaction

Reaction Comment Organism Reaction ID
L-histidine = urocanate + NH3 mechanism Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-fluorohistidine
-
 Pseudomonas sp. 2-fluorourocanate + NH3
-
 ?
4-fluoro-DL-histidine very poor substrate Pseudomonas sp. 4-fluorourocanate + NH3
-
 r
DL-histidine
-
 Pseudomonas sp. urocanate + NH3
-
 ?
L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
 r

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Pseudomonas sp.