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Literature summary for 4.3.1.28 extracted from

  • Khaw, L.; Boehm, G.; Metcalfe, S.; Staunton, J.; Leadlay, P.
    Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase (1998), J. Bacteriol., 180, 809-814.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10B cells Streptomyces hygroscopicus

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-
Streptomyces hygroscopicus NRRL 5491
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 Streptomyces hygroscopicus L-pipecolate + NH3
-
 ?
L-lysine
-
 Streptomyces hygroscopicus NRRL 5491 L-pipecolate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
lysine cyclodeaminase
-
 Streptomyces hygroscopicus
rapL
-
 Streptomyces hygroscopicus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Streptomyces hygroscopicus

General Information

General Information Comment Organism
malfunction introduction of a frameshift mutation into rapL gene gives rise to a mutant which does not produce significant amounts of rapamycin. Growth of this rapL mutant on media containing added L-pipecolate restores wild-type levels of rapamycin production Streptomyces hygroscopicus