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Literature summary for 4.3.1.28 extracted from

  • Gatto Jr., G.; Boyne II, M.; Kelleher, N.; Walsh, C.
    Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster (2006), J. Am. Chem. Soc., 128, 3838-3847.
    View publication on PubMed
Search for more literature for 4.3.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus

Inhibitors

Inhibitors Comment Organism Structure
(R)-(-)-nipecotic acid
-
 Streptomyces hygroscopicus
(S)-(+)-nipecotic acid
-
 Streptomyces hygroscopicus
thiazolidine-2-carboxylic acid
-
 Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 L-lysine 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 Streptomyces hygroscopicus L-pipecolate + NH3
-
 ?
L-ornithine the enzyme also accepts L-ornithine as a substrate, albeit with a significantly reduced catalytic efficiency Streptomyces hygroscopicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 40000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
lysine cyclodeaminase
-
 Streptomyces hygroscopicus
rapL
-
 Streptomyces hygroscopicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.61
-
 L-lysine 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme has about 0.17 equivalents of tightly bound NAD+. In the presence of exogenous NAD+, the initial rate is elevated 8fold with a Km of 0.0023 mM for the cofactor Streptomyces hygroscopicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.096
-
 in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus thiazolidine-2-carboxylic acid
0.68
-
 in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus (S)-(+)-nipecotic acid
4.8
-
 in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus (R)-(-)-nipecotic acid

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.14
-
 L-ornithine 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus
13
-
 L-lysine 0.1 mM NAD+, in 100 mM HEPES (pH 8.0), at 30°C Streptomyces hygroscopicus