Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stabilization of enzyme | Rhodotorula glutinis |
Application | Comment | Organism |
---|---|---|
synthesis | use of enzyme for synthesis of optically pure L-phenylalanine from trans-cinnamate | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
enzyme expression is induced up to 3fold by presence of L-isoleucine, L-phenylalanine or L-tyrosine in growth medium. The presence of trans-cinnamic acid suppresses enzyme expression | - |
Storage Stability | Organism |
---|---|
0-2°C, 50 mM Tris/HCl, 0.01% Mn2+, pH 8.8, whole cells retain 85% of initial enzyme activity for at least 12 weeks | Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
r | |
L-phenylalanine | - |
Rhodotorula glutinis RE4607095D | (E)-cinnamate + NH3 | - |
r | |
trans-cinnamate + NH3 | - |
Rhodotorula glutinis | L-phenylalanine | - |
r | |
trans-cinnamate + NH3 | - |
Rhodotorula glutinis RE4607095D | L-phenylalanine | - |
r |