Cloned (Comment) | Organism |
---|---|
controlled expression in Escherichia coli to mitigate product toxicity | Rhodotorula glutinis |
controlled expression in Escherichia coli to mitigate product toxicity | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
L-tyrosine | pH 9.5, 35°C | Phanerodontia chrysosporium | |
0.0677 | - |
L-tyrosine | pH 9.5 | Rhodotorula glutinis | |
0.126 | - |
L-phenylalanine | pH 9.5 | Rhodotorula glutinis | |
0.161 | - |
L-phenylalanine | pH 9.5, 35°C | Phanerodontia chrysosporium | |
0.183 | - |
L-phenylalanine | pH 8.5 | Rhodotorula glutinis | |
0.615 | - |
L-tyrosine | pH 8.5 | Rhodotorula glutinis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Rhodotorula glutinis | - |
- |
- |
Storage Stability | Organism |
---|---|
-80°C, 50 mM Tris, pH 8.5, 25% glycerol, stable | Phanerodontia chrysosporium |
4°C, 50 mM Tris, pH 8.5, stable for at least a month | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Phanerodontia chrysosporium | (E)-cinnamate + NH3 | - |
? | |
L-tyrosine | - |
Rhodotorula glutinis | p-hydroxycinnamic acid + NH3 | - |
? | |
L-tyrosine | - |
Phanerodontia chrysosporium | p-hydroxycinnamic acid + NH3 | - |
? | |
additional information | no substrate: L-histidine | Phanerodontia chrysosporium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 h, no loss of activity, 4 h, 72% residual activity | Phanerodontia chrysosporium |
60 | - |
4 h, 10% residual activity | Rhodotorula glutinis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
L-tyrosine | pH 8.5 | Rhodotorula glutinis | |
0.93 | - |
L-tyrosine | pH 9.5 | Rhodotorula glutinis | |
1.3 | - |
L-tyrosine | pH 9.5, 35°C | Phanerodontia chrysosporium | |
1.53 | - |
L-phenylalanine | pH 9.5 | Rhodotorula glutinis | |
1.6 | - |
L-phenylalanine | pH 8.5 | Rhodotorula glutinis | |
3.3 | - |
L-phenylalanine | pH 9.5, 35°C | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
35°C | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | above 88% of maximum activity | Phanerodontia chrysosporium |
10 | - |
80% of maximum activity | Phanerodontia chrysosporium |
12 | - |
12% of maximum activity | Phanerodontia chrysosporium |