Protein Variants | Comment | Organism |
---|---|---|
L137H | mutation almost doubles the kinetic D-isotope effect compared to wild-type enzyme | Petroselinum crispum |
L137H/Q487E | mutation almost doubles the kinetic D-isotope effect compared to wild-type enzyme | Petroselinum crispum |
Q487A | kinetic D isotope effect is of the same magnitude as wild-type enzyme | Petroselinum crispum |
Y350F | kinetic D isotope effect is of the same magnitude as wild-type enzyme | Petroselinum crispum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
L-phenylalanine | pH 8.8, 30°C, mutant enzyme Q487A | Petroselinum crispum | |
0.027 | - |
L-Phe | pH 8.8, 30°C, mutant enzyme Q487A | Petroselinum crispum | |
0.028 | - |
L-phenylalanine | pH 8.8, 30°C, mutant enzyme Y350F | Petroselinum crispum | |
0.033 | - |
L-Phe | pH 8.8, 30°C, mutant enzyme Y350F | Petroselinum crispum | |
9.8 | - |
L-phenylalanine | pH 8.8, 30°C, mutant enzyme L137H | Petroselinum crispum | |
14.2 | - |
L-Phe | pH 8.8, 30°C, mutant enzyme L137H | Petroselinum crispum | |
42.4 | - |
L-phenylalanine | pH 8.8, 30°C, mutant enzyme L137H/Q487E | Petroselinum crispum | |
75.6 | - |
L-Phe | pH 8.8, 30°C, mutant enzyme L137H/Q487E | Petroselinum crispum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | - |
recombinant wild-type enzyme and mutant enzymes Q487A, Y350F, and L137H | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe | - |
Petroselinum crispum | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Petroselinum crispum | trans-cinnamate + NH3 | - |
? |