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Literature summary for 4.3.1.2 extracted from

  • Asano, Y.; Kato, Y.; Levy, C.; Baker, P.; Rice, D.
    Structure and Function of Amino Acid Ammonia-lyases (2004), Biocatal. Biotransform., 22, 131-138.
No PubMed abstract available
Search for more literature for 4.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Citrobacter amalonaticus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Citrobacter amalonaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 mesaconic acid
-
 Citrobacter amalonaticus
0.76
-
 (2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus
7.9
-
 (2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
Cs+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus
K+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus
Mg2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
Mn2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
Rb+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000 Citrobacter freundii
42000
-
 2 * 42000 Citrobacter amalonaticus
44000
-
 2 * 44000 Morganella morganii
70000
-
 gel filtration Morganella morganii
79000
-
 gel filtration Citrobacter freundii
84000
-
 gel filtration Citrobacter amalonaticus

Organism

Organism UniProt Comment Textmining
Citrobacter amalonaticus
-
 YG-1002
-
Citrobacter amalonaticus YG-1002
-
 YG-1002
-
Citrobacter freundii
-
 YG.0504
-
Citrobacter freundii YG.0504
-
 YG.0504
-
Clostridium tetanomorphum
-
-
-
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Morganella morganii
-
 YG-0601
-
Morganella morganii YG-0601
-
 YG-0601
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus 2-chloro-2-butenedioic acid
-
 ?
(2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus YG-1002 2-chloro-2-butenedioic acid
-
 ?
(2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus mesaconate + NH3
-
 r
(2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
 r
additional information no reaction with aspartic acid Citrobacter amalonaticus ?
-
 ?
additional information no reaction with aspartic acid Citrobacter amalonaticus YG-1002 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000 Citrobacter amalonaticus
dimer 2 * 40000 Citrobacter freundii
dimer 2 * 44000 Morganella morganii

Synonyms

Synonyms Comment Organism
3-methylaspartate ammonia-lyase
-
 Citrobacter freundii
3-methylaspartate ammonia-lyase
-
 Clostridium tetanomorphum
3-methylaspartate ammonia-lyase
-
 Morganella morganii
3-methylaspartate ammonia-lyase
-
 Citrobacter amalonaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Citrobacter amalonaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 amination Citrobacter amalonaticus
9.7
-
 deamination Citrobacter amalonaticus