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Literature summary for 4.3.1.18 extracted from
- Rapid determination of free d-serine with chicken D-serine dehydratase (2011), J. Chromatogr. B, 879, 3326-3330.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a simple, rapid, and inexpensive method of measuring the concentration of intrinsic free D-serine in tissue samples. The method uses chicken D-serine dehydratase in an enzymatic reaction to produce pyruvate, which is detected spectrophotometrically. The presence of Zn2+ or EDTA dioes not have any effect on pyruvate formation under the present assay conditions. The method is not affected by the presence of a large excess of L-serine, nor by the presence of tissue extracts, and accurately determines concentrations of 2-30 microM of D-serine. The entire assay requires only 60 min | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 20 microM, increases pyruvate formation by 28% | Gallus gallus |  |
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