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Literature summary for 4.3.1.18 extracted from

  • Ito, T.; Takahashi, K.; Naka, T.; Hemmi, H.; Yoshimura, T.
    Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae (2007), Anal. Biochem., 371, 167-172.
    View publication on PubMed
Search for more literature for 4.3.1.18

Application

Application Comment Organism
medicine the established enzymatic method could be used for the quantitative determination of D-serine in human urine Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
into the pET15b vector for expression in Escherichia coli BL21DE3 cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-serine Saccharomyces cerevisiae
-
 pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-chelating DEAE-TOYOPEARL and a gel permeation column, from 1 l culture 8 mg Dsd1p is obtained Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine
-
 Saccharomyces cerevisiae pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
D-serine ammonia-lyase
-
 Saccharomyces cerevisiae
D-serine dehydratase
-
 Saccharomyces cerevisiae
DSD
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 activity assayed by the determination of the ammonia formend from D-serine Saccharomyces cerevisiae
37
-
 D-serine assay with the LDH-coupling method, the amount of released pyruvate is measured Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Saccharomyces cerevisiae