Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
General Stability | Organism |
---|---|
resolved completely by dialysis against L-Cys or D-Cys, reactivated by addition of pyridoxal 5'-phosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O-methylserine | competitive | Escherichia coli | |
Tris | inactivation is prevented by the presence of sufficient K+ or NH4+ and less effectively by Na+ or pyridoxal 5'-phosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
D-Ser | - |
Escherichia coli | |
3.2 | - |
D-Thr | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
constitutive mutant | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine | - |
Escherichia coli | pyruvate + NH3 | - |
? | |
D-threonine | - |
Escherichia coli | 2-oxobutanoate + NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 30% of maximal activity, pH 9.0: about 60% of maximal activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme linked cofactor | Escherichia coli |