Literature summary for 4.3.1.18 extracted from

Robinson, W.G.; Labow, R.
D-Serine dehydratase (Escherichia coli) (1971), Methods Enzymol., 17B, 356-360.

No PubMed abstract available

Search for more literature for 4.3.1.18

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Escherichia coli

General Stability

General Stability	Organism
repeated freezing and thawing over a period of 1 or 2 months causes a 20-30% loss of activity	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	D-Ser	-	Escherichia coli
0.92	-	D-Thr	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37300	-	analytical ultracentrifugation	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Storage Stability

Storage Stability	Organism
0°C, stable almost indefinitely	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-serine	-	Escherichia coli	pyruvate + NH3	-	?
D-threonine	-	Escherichia coli	2-oxobutanoate + NH3	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
22	-	6 h, 10% loss of activity	Escherichia coli
54	-	1 min, dilute solution, complete loss of activity	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	1 mol of pyridoxal 5'-phosphate is bound per mol of enzyme to one epsilon-amino group	Escherichia coli

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Release 2023.1 (January 2023)