Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 DE3 | Legionella pneumophila |
Crystallization (Comment) | Organism |
---|---|
- |
Legionella pneumophila |
Protein Variants | Comment | Organism |
---|---|---|
C458A | kcat/Km for L-serine is 2.2fold lower than the wild-type value | Legionella pneumophila |
DELTA(C458) | mutant enzyme has no detectable activity | Legionella pneumophila |
DELTA(P456,E457,C458) | mutant enzyme has no detectable activity | Legionella pneumophila |
E445A | mutant enzyme has no detectable activity | Legionella pneumophila |
E457A | kcat/Km for L-serine is 1.5fold lower than the wild-type value | Legionella pneumophila |
H124A/N126A | kcat/Km for L-serine is 48.5fold lower than the wild-type value | Legionella pneumophila |
H19A | kcat/Km for L-serine is 127fold lower than the wild-type value | Legionella pneumophila |
H61A | mutant enzyme has no detectable activity | Legionella pneumophila |
H61N | mutant enzyme has no detectable activity | Legionella pneumophila |
H61S | mutant enzyme has no detectable activity | Legionella pneumophila |
K444A | mutant enzyme has no detectable activity | Legionella pneumophila |
S16A | mutant enzyme has no detectable activity | Legionella pneumophila |
S17A | kcat/Km for L-serine is 4.8fold lower than the wild-type value | Legionella pneumophila |
S18A | mutant enzyme has no detectable activity | Legionella pneumophila |
S53A | kcat/Km for L-serine is 2.9fold lower than the wild-type value | Legionella pneumophila |
T20A | kcat/Km for L-serine is 4.2fold lower than the wild-type value | Legionella pneumophila |
T290A | kcat/Km for L-serine is 127fold lower than the wild-type value | Legionella pneumophila |
T446A | kcat/Km for L-serine is 91.7fold lower than the wild-type value | Legionella pneumophila |
T57A | kcat/Km for L-serine is 31.7fold lower than the wild-type value | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T57A | Legionella pneumophila | |
2 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S17A | Legionella pneumophila | |
2 | - |
L-serine | pH 7.0, temperature not specified in the publication, native enzyme | Legionella pneumophila | |
2.3 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme E457A | Legionella pneumophila | |
3 | 3.2 | L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A | Legionella pneumophila | |
3.1 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T290A | Legionella pneumophila | |
3.5 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S53A | Legionella pneumophila | |
4.7 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T20A | Legionella pneumophila | |
6.9 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme C458A | Legionella pneumophila | |
24.4 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H19A | Legionella pneumophila | |
189 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T446A | Legionella pneumophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Legionella pneumophila | - |
pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | Q5ZXE1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Legionella pneumophila | pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-serine dehydratase | - |
Legionella pneumophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.07 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T290A | Legionella pneumophila | |
8.8 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T57A | Legionella pneumophila | |
31 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H19A | Legionella pneumophila | |
67 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S17A | Legionella pneumophila | |
112 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A | Legionella pneumophila | |
181 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T20A | Legionella pneumophila | |
201 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S53A | Legionella pneumophila | |
249 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme E457A | Legionella pneumophila | |
330 | - |
L-serine | pH 7.0, temperature not specified in the publication, native enzyme | Legionella pneumophila | |
356 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T446A | Legionella pneumophila | |
512 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme C458A | Legionella pneumophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H19A | Legionella pneumophila | |
1.3 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T290A | Legionella pneumophila | |
1.8 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T446A | Legionella pneumophila | |
3.4 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A | Legionella pneumophila | |
5.2 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T57A | Legionella pneumophila | |
34 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S17A | Legionella pneumophila | |
39 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme T20A | Legionella pneumophila | |
57 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S53A | Legionella pneumophila | |
74 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme C458A | Legionella pneumophila | |
108 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme E457A | Legionella pneumophila | |
165 | - |
L-serine | pH 7.0, temperature not specified in the publication, mutant enzyme S16A | Legionella pneumophila |