Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structure of A65S hSDH mutant is determined at 1.3 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A65S | structure of A65S hSDH mutant is determined at 1.3 A resolution. Mutant shows decreased activity (50%) with substrate L-serine compared to wild-type. Mutant shows measurable activity with substrate D-serine | Homo sapiens |
A65S/V225S | double mutant shows only 10% of activity with substrate L-serine compared to wild-type (100%). Double mutant shows very little but measurable activity with substrate D-serine | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P20132 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | wild-type enzyme shows almost no activity with D-serine | Homo sapiens | pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HSDH | - |
Homo sapiens |
Serine dehydratase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |