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Literature summary for 4.3.1.17 extracted from

  • Wang, C.Y.; Ku, S.C.; Lee, C.C.; Wang, A.H.
    Modulating the function of human serine racemase and human serine dehydratase by protein engineering (2012), Protein Eng. Des. Sel., 25, 741-749.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of A65S hSDH mutant is determined at 1.3 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
A65S structure of A65S hSDH mutant is determined at 1.3 A resolution. Mutant shows decreased activity (50%) with substrate L-serine compared to wild-type. Mutant shows measurable activity with substrate D-serine Homo sapiens
A65S/V225S double mutant shows only 10% of activity with substrate L-serine compared to wild-type (100%). Double mutant shows very little but measurable activity with substrate D-serine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P20132
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine wild-type enzyme shows almost no activity with D-serine Homo sapiens pyruvate + NH3
-
?

Synonyms

Synonyms Comment Organism
HSDH
-
Homo sapiens
Serine dehydratase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens