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Literature summary for 4.3.1.17 extracted from

  • Ogawa, H.; Gomi, T.; Nishizawa, M.; Hayakawa, Y.; Endo, S.; Hayashi, K.; Ochiai, H.; Takusagawa, F.; Pitot, H.C.; Mori, H.; Sakurai, H.; Koizumi, K.; Saiki, I.; Oda, H.; Fujishita, T.; Miwa, T.; Maruyama, M.; Kobayashi, M.
    Enzymatic and biochemical properties of a novel human serine dehydratase isoform (2006), Biochim. Biophys. Acta, 1764, 961-971.
    View publication on PubMed
Search for more literature for 4.3.1.17

Cloned(Commentary)

Cloned (Comment) Organism
the cSDH sequence is cloned into the pCW and pET28a vector for expression of the protein in Escherichia coli BL21DE3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
cSDH-hSDH chimeric protein Homo sapiens
DELTAP128 hSDH Homo sapiens
InsP128 cSDH, cSDH lacks a Pro residue corresponding to Pro128 in hSDH Homo sapiens
L287V cSDH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
 threonine cSDH Homo sapiens
3.5
-
 threonine cSDH +Pro Homo sapiens
4.2
-
 threonine cSDH L287V Homo sapiens
13.2
-
 threonine cSDH-hSDH Homo sapiens
14.9
-
 threonine hSDH -Pro Homo sapiens
30.7
-
 serine cSDH L287V Homo sapiens
32.2
-
 serine cSDH Homo sapiens
34.5
-
 serine cSDH +Pro Homo sapiens
35.7
-
 serine hSDH -Pro Homo sapiens
44.4
-
 serine cSDH-hSDH Homo sapiens
59.5
-
 threonine hSDH Homo sapiens
67.3
-
 serine hSDH Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34630
-
 hSDH, calculated molecular weight Homo sapiens
34670
-
 cSDH, calculated molecular weight Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Homo sapiens
-
 pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P20132
-
-

Purification (Commentary)

Purification (Comment) Organism
cSDH is purified using a Sephacryl S-200 and a Ni2+-NAT column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain cancerous SDH Homo sapiens
-
hepatocyte hepatic SDH Homo sapiens
-
kidney cancerous SDH Homo sapiens
-
lung cancerous SDH Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Homo sapiens pyruvate + NH3
-
 ?
L-threonine
-
 Homo sapiens alpha-ketobutyrate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
cSDH cancerous SDH Homo sapiens
HSDH hepatic SDH Homo sapiens
L-serine ammonia-lyase
-
 Homo sapiens
SDH
-
 Homo sapiens
Serine dehydratase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 activity assay Homo sapiens