General Stability | Organism |
---|---|
it is absolutely critical that dilutions of the purified enzyme be made in buffer containing 50% glycerol, otherwise the enzyme rapidly loses activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-serine | competitive | Escherichia coli | |
L-alanine | - |
Escherichia coli | |
L-cysteine | competitive | Escherichia coli | |
additional information | not inhibitory: L-alanine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.67 | - |
L-serine | pH 8.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | presence of (4Fe-4S)2+ and of (2Fe-2S)2+ clusters involved in catalytic turnover | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, His-tagged protein, SDS-PAGE and calculated | Escherichia coli |
107000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, purification under anaerobic conditions | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-threonine | at 4.6% of the rate with L-serine | Escherichia coli | 2-oxobutyrate + NH3 | - |
? | |
L-serine | - |
Escherichia coli | pyruvate + NH3 | - |
? | |
L-threonine | at 2.7% of the rate with L-serine | Escherichia coli | 2-oxobutyrate + NH3 | - |
? | |
additional information | no substrate: D-serine. Extremely poor substrate: L-cysteine | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000, His-tagged protein, SDS-PAGE and calculated | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
436 | - |
L-serine | pH 8.0, 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-alanine | pH 8.0, 37°C | Escherichia coli | |
1.41 | - |
D-serine | pH 8.0, 37°C | Escherichia coli |