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Literature summary for 4.3.1.17 extracted from

  • Hofmeister, A.E.M.; Grabowski, R.; Linder, D.; Buckel, W.
    L-Serine and L-threonine dehydratase from Clostridium propionicum. Two enzymes with different prosthetic groups (1993), Eur. J. Biochem., 215, 341-349.
    View publication on PubMed
Search for more literature for 4.3.1.17

General Stability

General Stability Organism
extreme instability does not permit purification to homogeneity Anaerotignum propionicum

Inhibitors

Inhibitors Comment Organism Structure
D-Ser
-
 Anaerotignum propionicum
L-Cys
-
 Anaerotignum propionicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
 L-Ser
-
 Anaerotignum propionicum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur-dependent enzyme Anaerotignum propionicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 1 * 26000 + 1 * 10000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum
26000
-
 1 * 26000 + 1 * 10000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum
26000
-
 4 * 26000 + 4 * 30000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum
30000
-
 4 * 26000 + 4 * 30000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum
57000
-
 gel filtration in presence of 10 mM Fe2+, dimeric enzyme form Anaerotignum propionicum
230000
-
 gel filtration in absence of Fe2+, octameric enzyme form Anaerotignum propionicum

Organism

Organism UniProt Comment Textmining
Anaerotignum propionicum
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid loss of activity upon exposure to air, reactivation by Fe2+ Anaerotignum propionicum

Purification (Commentary)

Purification (Comment) Organism
partial Anaerotignum propionicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Anaerotignum propionicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Anaerotignum propionicum pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 26000 + 1 * 10000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum
octamer 4 * 26000 + 4 * 30000, enzyme either exists as a heterooctamer or a heterodimer, SDS-PAGE Anaerotignum propionicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate pyridoxal-5'-phosphate-independent enzyme Anaerotignum propionicum