Crystallization (Comment) | Organism |
---|---|
crystal structure of DAPAL from Escherichia coli (EcDAPAL) in tetragonal and monoclinic forms at 2.0 and 2.2 A resolutions is shown | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D120N | mutant shows no reaction with D-DAP, Km (L-DAP) increased compared to wild-type, kcat decreased | Escherichia coli |
D189N | Km (D-DAP) and (L-DAP) increased compared to wild-type, kcat decreased | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli | |
0.05 | - |
D-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli | |
0.57 | - |
L-2,3-diaminopropanoate | mutant D120N, pH and temperature not specified in the publication | Escherichia coli | |
11.5 | - |
L-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli | |
12.9 | - |
D-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43300 | - |
calculated from cDNA | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P66899 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2,3-Diaminopropanoate + H2O | - |
Escherichia coli | Pyruvate + NH3 | - |
? | |
L-2,3-Diaminopropanoate + H2O | - |
Escherichia coli | Pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EcDAPAL | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli | |
0.35 | - |
D-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli | |
3.05 | - |
L-2,3-diaminopropanoate | mutant D120N, pH and temperature not specified in the publication | Escherichia coli | |
24.6 | - |
L-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli | |
44.58 | - |
D-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
L-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli | |
0.0266 | - |
D-2,3-diaminopropanoate | mutant D189N, pH and temperature not specified in the publication | Escherichia coli | |
5.35 | - |
L-2,3-diaminopropanoate | mutant D120N, pH and temperature not specified in the publication | Escherichia coli | |
820 | - |
L-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli | |
890 | - |
D-2,3-diaminopropanoate | wild-type, pH and temperature not specified in the publication | Escherichia coli |