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Literature summary for 4.3.1.15 extracted from

  • Bisht, S.; Rajaram, V.; Bharath, S.R.; Kalyani, J.N.; Khan, F.; Rao, A.N.; Savithri, H.S.; Murthy, M.R.
    Crystal structure of Escherichia coli diaminopropionate ammonia-lyase reveals mechanism of enzyme activation and catalysis (2012), J. Biol. Chem., 287, 20369-20381.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of DAPAL from Escherichia coli (EcDAPAL) in tetragonal and monoclinic forms at 2.0 and 2.2 A resolutions is shown Escherichia coli

Protein Variants

Protein Variants Comment Organism
D120N mutant shows no reaction with D-DAP, Km (L-DAP) increased compared to wild-type, kcat decreased Escherichia coli
D189N Km (D-DAP) and (L-DAP) increased compared to wild-type, kcat decreased Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
L-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli
0.05
-
D-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli
0.57
-
L-2,3-diaminopropanoate mutant D120N, pH and temperature not specified in the publication Escherichia coli
11.5
-
L-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli
12.9
-
D-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
calculated from cDNA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P66899
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2,3-Diaminopropanoate + H2O
-
Escherichia coli Pyruvate + NH3
-
?
L-2,3-Diaminopropanoate + H2O
-
Escherichia coli Pyruvate + NH3
-
?

Subunits

Subunits Comment Organism
homodimer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
EcDAPAL
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
L-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli
0.35
-
D-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli
3.05
-
L-2,3-diaminopropanoate mutant D120N, pH and temperature not specified in the publication Escherichia coli
24.6
-
L-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli
44.58
-
D-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
L-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli
0.0266
-
D-2,3-diaminopropanoate mutant D189N, pH and temperature not specified in the publication Escherichia coli
5.35
-
L-2,3-diaminopropanoate mutant D120N, pH and temperature not specified in the publication Escherichia coli
820
-
L-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli
890
-
D-2,3-diaminopropanoate wild-type, pH and temperature not specified in the publication Escherichia coli