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Literature summary for 4.3.1.15 extracted from

  • Kalyani, J.N.; Ramachandra, N.; Kachroo, A.H.; Mahadevan, S.; Savithri, H.S.
    Functional analysis of the genes encoding diaminopropionate ammonia lyase in Escherichia coli and Salmonella enterica serovar Typhimurium (2012), J. Bacteriol., 194, 5604-5612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
C271V Km for DL-DAP is 47fold higher compared to wild-type and kcat decreased by 1.5fold compared to wild-type.In contrast to wild-type C271V sDAPAL exhibits only 2fold stimulation in activity even when a high concentration of KCl is used Salmonella enterica subsp. enterica serovar Typhimurium
C299V kcat and Km for DL-DAP are similar to wild-type Salmonella enterica subsp. enterica serovar Typhimurium
D125E mutant does not show any activity with D-DAP at all Salmonella enterica subsp. enterica serovar Typhimurium
D125S kcat value in mutant is reduced by 5.4fold for D-DAP compared to wild-type Salmonella enterica subsp. enterica serovar Typhimurium
D194P mutant does not show any activity with either L-DAP or D-DAP. Kd for D-DAP shows a 5fold increase in mutant compared to wild-type. L-DAP does not bind at all to mutant D194P Salmonella enterica subsp. enterica serovar Typhimurium
D194S mutant exhibits an 16fold decrease in kcat with L-DAP whereas activity with D-DAP is reduced only by factor 1.7 compared to wild-type Salmonella enterica subsp. enterica serovar Typhimurium
T385D kcat value with DL-DAP as substrate is 69% of wild-type. Km value for D-Ser doubled in T385D mutant whereas the kcat value increased by 7fold Salmonella enterica subsp. enterica serovar Typhimurium
T385S kcat value with DL-DAP as substrate is 86% of wild-type. Mutant exhibits a 2fold higher Kcat with D-Ser compared to wild-type Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant C271V sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.246
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.279
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, untagged sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.282
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.284
-
 L-cysteine pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.293
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, mutant D194S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.3
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.3
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.33
-
 D-serine pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.346
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.375
-
 D-serine pH 7.4, 30°C, mutant T385S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.378
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D194S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.382
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant T385S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.412
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125E sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.43
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant C299V sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.52
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
0.6
-
 D-serine pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
KCl addition of increasing concentrations (higher than 10 mM) enhances the activity by 10fold Salmonella enterica subsp. enterica serovar Typhimurium
NaCl addition of increasing concentrations enhances the activity by 2fold Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Escherichia coli P66899
-
-
Salmonella enterica P40817
-
-
Salmonella enterica subsp. enterica serovar Typhimurium P40817
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2,3-diaminopropanoate + 2 H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + 2 NH3
-
 ?
D-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
DL-2,3-diaminopropanoate + 2 H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + 2 NH3
-
 ?
L-2,3-diaminopropanoate + 2 H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + 2 NH3
-
 ?
L-cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

Synonyms Comment Organism
DAPAL
-
 Escherichia coli
DAPAL
-
 Salmonella enterica
diaminopropionate ammonia lyase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
diaminopropionate ammonia lyase
-
 Escherichia coli
diaminopropionate ammonia lyase
-
 Salmonella enterica
sDAPAL
-
 Salmonella enterica subsp. enterica serovar Typhimurium
STM1002
-
 Salmonella enterica
ygeX
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 L-cysteine pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
2.67
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D194S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
5.17
-
 D-serine pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
7.83
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
10.5
-
 D-serine pH 7.4, 30°C, mutant T385S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
20.83
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125E sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
26
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, mutant D194S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
34
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant C271V sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
34.17
-
 D-serine pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
37.33
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, mutant D125S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
42
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant T385D sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
42.83
-
 L-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
51.67
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant T385S sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
57
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, mutant C299V sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
60.17
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
69.17
-
 DL-2,3-diaminopropanoate pH 7.4, 30°C, untagged sDAPL Salmonella enterica subsp. enterica serovar Typhimurium
375.8
-
 D-2,3-diaminopropanoate pH 7.4, 30°C, sDAPL Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
pyridoxal 5'-phosphate
-
 Escherichia coli
pyridoxal 5'-phosphate
-
 Salmonella enterica

Expression

Organism Comment Expression
Escherichia coli low expression of ygeX gene is shown by RT-PCR down

General Information

General Information Comment Organism
malfunction deletion shows loss of growth on DL-DAP, confirming that STM1002 is the locus encoding DAPAL. Inhibition by DL-DAP is rescued by transforming the strains with plasmids containing the STM1002 gene encoding DAPAL or supplementing the medium with casamino acids Salmonella enterica
malfunction deletion shows loss of growth on DL-DAP, confirming that ygeX is the locus encoding DAPAL. Inhibition by DL-DAP is rescued by transforming the strains with plasmids containing the ygeX gene encoding DAPAL or supplementing the medium with casamino acids Escherichia coli