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Literature summary for 4.3.1.12 extracted from

  • Schindler, U.; Sans, N.; Schroeder, J.
    Ornithine cyclodeaminase from octopine Ti plasmid Ach5: identification, DNA sequence, enzyme properties, and comparison with gene and enzyme from nopaline Ti plasmid C58 (1989), J. Bacteriol., 171, 847-854.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens
OCD, Arch5, and OCD, C58 Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Agrobacterium tumefaciens
0.25
-
 L-ornithine OCD, C58, in the presence of L-arginine Agrobacterium tumefaciens
0.25
-
 L-ornithine OCD, Ach5, in the absence of L-arginine Agrobacterium tumefaciens
1.7
-
 L-ornithine in the absence of L-arginine , OCD, C58 Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 2 * 39500, OCD, Ach5, calculation from sequence of amino acid Agrobacterium tumefaciens
42000
-
 2 * 42000, OCD, Ach5, SDS-PAGE Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine Agrobacterium tumefaciens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
 C58, pTi nopaline
-
Agrobacterium tumefaciens
-
 Ach5, pTi octopine
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Agrobacterium tumefaciens

Storage Stability

Storage Stability Organism
-20°C, OCD, Arch5, and OCD, C58, stable for several months without loss of activity Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
 Agrobacterium tumefaciens L-proline + NH3
-
 ?
L-ornithine
-
 Agrobacterium tumefaciens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39500, OCD, Ach5, calculation from sequence of amino acid Agrobacterium tumefaciens
dimer 2 * 42000, OCD, Ach5, SDS-PAGE Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 35 OCD, C58 Agrobacterium tumefaciens
25 35 in the absence of L-arginine Agrobacterium tumefaciens
30 35 OCD, Ach5 Agrobacterium tumefaciens
38 42 OCD, C58 Agrobacterium tumefaciens
38 42 in the presence of L-arginine Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 OCD, Ach5 Agrobacterium tumefaciens
8 9.5 in the absence of L-arginine Agrobacterium tumefaciens
8 9.5 OCD, C58 Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NAD+ required for maximal activity Agrobacterium tumefaciens