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Literature summary for 4.3.1.10 extracted from

  • Tudball, N.; Thomas, P.; Bailey-Wood, R.
    The purification and properties of the L-serine O-sulphate degrading system of pig liver (1971), Biochem. J., 121, 747-752.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.10

General Stability

General Stability Organism
complete loss of activity if enzyme-containing solution is frozen and thawed Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26
-
 L-serine O-sulfate alpha enzyme Sus scrofa
26
-
 L-serine O-sulfate beta enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration, beta enzyme Sus scrofa
52000
-
 gel filtration, alpha enzyme Sus scrofa
53800
-
 high-speed membrane osmometry, alpha enzyme Sus scrofa
54500
-
 high-speed membrane osmometry, beta enzyme Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
4°C, 10% loss of activity after 1 week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine O-sulfate optimum activity at a concentration of 0.05 M Sus scrofa pyruvate + NH3 + sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 enzyme assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Sus scrofa