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Literature summary for 4.3.1.1 extracted from

  • Wang, Z.W.; Chen, Y.; Chao, Y.P.
    Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase (2006), J. Biotechnol., 124, 403-411.
    View publication on PubMed

Application

Application Comment Organism
biotechnology enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase. The excretion of acetate by the aerobic growth of Escherichia coli on glucose is a manifestation of imbalanced flux between glycolysis and the tricarboxylic acid (TCA) cycle. This may restrict the production of recombinant proteins in E. coli, due to the limited amounts of precursor metabolites produced in TCA cycle. To approach this issue, an extra supply of intermediate metabolites in TCA cycle is made by conversion of aspartate to fumarate, a reaction mediated by the activity of L-aspartate ammonia-lyase Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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