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Literature summary for 4.3.1.1 extracted from

  • Yoon, M-Y.; Park, J.H.; Choi, K.J.; Kim, J.M.; Kim, Y.O.; Park, J.B.; Kyung, J.B.
    Purification and characterization of aspartase from Hafnia alvei (1998), J. Biochem. Mol. Biol., 31, 345-349.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 18% loss of activity Hafnia alvei
Zn2+ 0.0005 mM, 40% loss of activity Hafnia alvei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required Hafnia alvei
Mg2+ activates at the alkine pH Hafnia alvei
Mn2+ required at alkine pH Hafnia alvei
additional information
-
Hafnia alvei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Hafnia alvei
173000
-
sedimentation equilibrium analysis Pseudomonas fluorescens
198000
-
pore gradient polyacrylamide Hafnia alvei

Organism

Organism UniProt Comment Textmining
Hafnia alvei
-
ATCC 9760
-
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hafnia alvei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
236.8
-
-
Hafnia alvei

Storage Stability

Storage Stability Organism
-20°C, stable for a few weeks Hafnia alvei
-70°C stable over 3 months Hafnia alvei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate r Pseudomonas fluorescens fumarate + NH3
-
?
L-aspartate r Hafnia alvei fumarate + NH3
-
?
L-aspartate natural substrate Pseudomonas fluorescens fumarate + NH3
-
?
L-aspartate natural substrate Hafnia alvei fumarate + NH3
-
?

Subunits

Subunits Comment Organism
tetramer
-
Pseudomonas fluorescens
tetramer 4 * 55000, SDS-PAGE Hafnia alvei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
maximum activity Hafnia alvei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 60 15C: 90% loss of maximal activity, 60C: 90% loss of activity Hafnia alvei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in the absence of MgCl2 Hafnia alvei
8.5
-
in the presence of 20 mM MgCl2 Hafnia alvei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9
-
Hafnia alvei