Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 18% loss of activity | Hafnia alvei | |
Zn2+ | 0.0005 mM, 40% loss of activity | Hafnia alvei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required | Hafnia alvei | |
Mg2+ | activates at the alkine pH | Hafnia alvei | |
Mn2+ | required at alkine pH | Hafnia alvei | |
additional information | - |
Hafnia alvei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Hafnia alvei |
173000 | - |
sedimentation equilibrium analysis | Pseudomonas fluorescens |
198000 | - |
pore gradient polyacrylamide | Hafnia alvei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hafnia alvei | - |
ATCC 9760 | - |
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hafnia alvei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
236.8 | - |
- |
Hafnia alvei |
Storage Stability | Organism |
---|---|
-20°C, stable for a few weeks | Hafnia alvei |
-70°C stable over 3 months | Hafnia alvei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | r | Pseudomonas fluorescens | fumarate + NH3 | - |
? | |
L-aspartate | r | Hafnia alvei | fumarate + NH3 | - |
? | |
L-aspartate | natural substrate | Pseudomonas fluorescens | fumarate + NH3 | - |
? | |
L-aspartate | natural substrate | Hafnia alvei | fumarate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Pseudomonas fluorescens |
tetramer | 4 * 55000, SDS-PAGE | Hafnia alvei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
maximum activity | Hafnia alvei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | 15C: 90% loss of maximal activity, 60C: 90% loss of activity | Hafnia alvei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in the absence of MgCl2 | Hafnia alvei |
8.5 | - |
in the presence of 20 mM MgCl2 | Hafnia alvei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Hafnia alvei |