Literature summary for 4.2.99.B1 extracted from

Caglayan, M.; Batra, V.K.; Sassa, A.; Prasad, R.; Wilson, S.H.
Role of polymerase beta in complementing aprataxin deficiency during abasic-site base excision repair (2014), Nat. Struct. Mol. Biol., 21, 497-499.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of isoform POLB in complex with nicked DNA that represents the 5'-adenylated deoxyribose phosphate-containing base excision repair intermediate. The 5'-AMP-deoxyribose phosphate group is positioned in the lyase active site	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K68A	mutation has no significant effect on the removal of 5'-AMP-deoxyribose phosphate	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06746	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	in addition to removal of 5'-deoxyribose phosphate from base excision repair intermediates, enzyme also removes 5'-adenylated deoxyribose phosphate from base excision repair intermediates after abortive ligation	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
DNA polymerase beta	-	Homo sapiens
PolB	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	isoform POLB expression rescues methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast. Model suggests that aprataxin hydrolyzes the 5'-AMP group and allows for renewed attempts at processing of the repair intermediate, and the 5'-deoxyribose phosphate may be channeled into the normal base excision repair pathway for isoform POLB deoxyribose phsosphate removal and gap filling. In long-path base excision repair, the blocking intermediates can be processed via flap excision by FEN1. In an alternative mechanism, POLB could play a part by direct removal of the entire 5'-AMP-deoxyribose phosphate blocking group via its deoxyribose phosphate lyase activity	Homo sapiens

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Release 2023.1 (January 2023)