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Literature summary for 4.2.99.22 extracted from

  • Nomura, T.; Ogita, S.; Kato, Y.
    A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in tulip (2012), Plant Physiol., 159, 565-578.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tulipa gesneriana

Protein Variants

Protein Variants Comment Organism
D327N 0.1% of wild-type activity Tulipa gesneriana
G152A 0.1% of wild-type activity Tulipa gesneriana
G153A 0.01% of wild-type activity Tulipa gesneriana
G154A 88% of wild-type activity Tulipa gesneriana
H359A 0.001% of wild-type activity Tulipa gesneriana
H362A 6% of wild-type activity Tulipa gesneriana
S235A 0.01% of wild-type activity Tulipa gesneriana

Inhibitors

Inhibitors Comment Organism Structure
NaF 0.2 mM, 80% inhibition Tulipa gesneriana
phenylmethylsulfonyl fluoride 2 mM, 91% inhibition Tulipa gesneriana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18
-
 6-tuliposide A pH 6.5, 22°C Tulipa gesneriana
71
-
 6-tuliposide B pH 6.5, 22°C Tulipa gesneriana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Tulipa gesneriana 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Tulipa gesneriana
85400
-
 gel filtration Tulipa gesneriana

Organism

Organism UniProt Comment Textmining
Tulipa gesneriana I4DST8 isoform TCEA1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tulipa gesneriana

Source Tissue

Source Tissue Comment Organism Textmining
additional information gene TCEA1 is transcribed in all tissues except for bulbs Tulipa gesneriana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2260
-
 pH 6.5, 22°C Tulipa gesneriana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-tuliposide A
-
 Tulipa gesneriana tulipalin A + D-glucose enzyme accepts 6-tuliposide A to catalyze only the intramolecular transesterification but not the hydrolysis ?
6-tuliposide B 8.7% of the activity with 6-tuliposide A Tulipa gesneriana tulipalin B + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Tulipa gesneriana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 45
-
 Tulipa gesneriana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to, for 30 min Tulipa gesneriana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
510
-
 6-tuliposide B pH 6.5, 22°C Tulipa gesneriana
2800
-
 6-tuliposide A pH 6.5, 22°C Tulipa gesneriana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Tulipa gesneriana

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 when maintained at room temperature Tulipa gesneriana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.2
-
 6-tuliposide A pH 6.5, 22°C Tulipa gesneriana
160
-
 6-tuliposide A pH 6.5, 22°C Tulipa gesneriana