KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00079 | - |
isochorismate | substrate 2-2H-isochorismate, pH 7.5, 30°C | Pseudomonas aeruginosa | |
0.00105 | - |
isochorismate | pH 7.5, 30°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? | |
additional information | the 2H kinetic isotope effects on kcat and the ratio kcat/Km are 2.34 and 1.75, respectively. Chemistry is significantly rate-determining for the enzyme. The magnitude of the isotope effect is consistent with considerable C-H bond cleavage in the transition state. The significant 2H kinetic isotope effect and quantitative transfer of the label to pyruvate are both consistent with a pericyclic reaction mechanism | Pseudomonas aeruginosa | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
isochorismate | substrate 2-2H-isochorismate, pH 7.5, 30°C | Pseudomonas aeruginosa | |
1 | - |
isochorismate | pH 7.5, 30°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
549 | - |
isochorismate | substrate 2-2H-isochorismate, pH 7.5, 30°C | Pseudomonas aeruginosa | |
962 | - |
isochorismate | pH 7.5, 30°C | Pseudomonas aeruginosa |