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Literature summary for 4.2.99.21 extracted from

  • DeClue, M.S.; Baldridge, K.K.; Kunzler, D.E.; Kast, P.; Hilvert, D.
    Isochorismate pyruvate lyase: a pericyclic reaction mechanism? (2005), J. Am. Chem. Soc., 127, 15002-15003.
    View publication on PubMed
Search for more literature for 4.2.99.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00079
-
 isochorismate substrate 2-2H-isochorismate, pH 7.5, 30°C Pseudomonas aeruginosa
0.00105
-
 isochorismate pH 7.5, 30°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
 Pseudomonas aeruginosa salicylate + pyruvate
-
 ?
additional information the 2H kinetic isotope effects on kcat and the ratio kcat/Km are 2.34 and 1.75, respectively. Chemistry is significantly rate-determining for the enzyme. The magnitude of the isotope effect is consistent with considerable C-H bond cleavage in the transition state. The significant 2H kinetic isotope effect and quantitative transfer of the label to pyruvate are both consistent with a pericyclic reaction mechanism Pseudomonas aeruginosa ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
 isochorismate substrate 2-2H-isochorismate, pH 7.5, 30°C Pseudomonas aeruginosa
1
-
 isochorismate pH 7.5, 30°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
549
-
 isochorismate substrate 2-2H-isochorismate, pH 7.5, 30°C Pseudomonas aeruginosa
962
-
 isochorismate pH 7.5, 30°C Pseudomonas aeruginosa