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Literature summary for 4.2.99.21 extracted from

  • Kunzler, D.E.; Sasso, S.; Gamper, M.; Hilvert, D.; Kast, P.
    Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection (2005), J. Biol. Chem., 280, 32827-32834.
    View publication on PubMed
Search for more literature for 4.2.99.21

Protein Variants

Protein Variants Comment Organism
Q91N 20fold decrease in both isochorismate pyruvate lyase and chorismate mutase activity Pseudomonas aeruginosa
R54K 100fold decrease in both isochorismate pyruvate lyase and chorismate mutase activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 enzyme possesses isochorismate pyruvate lyase and weak chorismate mutase activity
-

Reaction

Reaction Comment Organism Reaction ID
isochorismate = salicylate + pyruvate [1,5]-sigmatropic reaction mechanism that invokes electrostatic catalysis in analogy to the [3,3]-pericyclic rearrangement of chorismate in chorismate mutase Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
 Pseudomonas aeruginosa salicylate + pyruvate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
0.27
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
1.06
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
 isochorismate mutant R54K, pH 7.5, 30°C Pseudomonas aeruginosa
57
-
 isochorismate mutant Q91N, pH 7.5, 30°C Pseudomonas aeruginosa
1130
-
 isochorismate wild-type, pH 7.5, 30°C Pseudomonas aeruginosa