Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum aerophilum |
Protein Variants | Comment | Organism |
---|---|---|
D172N | binds to 8-oxoguanine containing single-stranded DNA more tightly than double-stranded DNA containing a 8-oxoguanine/cytosine base pair | Pyrobaculum aerophilum |
D172Q | binds to 8-oxoguanine containing single-stranded DNA more tightly than double-stranded DNA containing a 8-oxoguanine/cytosine base pair | Pyrobaculum aerophilum |
D172Q | no detectable glycosylase activity | Pyrobaculum aerophilum |
K140Q | binds to 8-oxoguanine containing single-stranded DNA more tightly than double-stranded DNA containing a 8-oxoguanine/cytosine base pair | Pyrobaculum aerophilum |
Q31E/D218S | almost no glycosylase activity | Pyrobaculum aerophilum |
Q31S | significantly reduced glycosylase activity | Pyrobaculum aerophilum |
W222A | almost no glycosylase activity | Pyrobaculum aerophilum |
W222F | mutant is similarly active as wild type on 8-oxoguanine/C substrates | Pyrobaculum aerophilum |
W69F | mutant is similarly active as wild type on 8-oxoguanine/C substrates | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZVK6 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZVK6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA with an abasic site | - |
Pyrobaculum aerophilum | ? | - |
? | |
DNA with an abasic site | - |
Pyrobaculum aerophilum DSM 7523 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pa-AGOG DNA glycosylase | bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity) | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
Tm-value for mutant enzyme W69F | Pyrobaculum aerophilum |
84 | - |
Tm-value for mutant enzyme D172Q | Pyrobaculum aerophilum |
86 | - |
Tm-value for mutant enzyme Q31S | Pyrobaculum aerophilum |
87 | - |
Tm-value for mutant enzyme Q31E/D218S | Pyrobaculum aerophilum |
87 | - |
Tm-value for mutant enzyme W222A | Pyrobaculum aerophilum |
88 | - |
Tm-value for mutant enzyme W222F | Pyrobaculum aerophilum |
93 | - |
Tm-value for wild-type enzyme | Pyrobaculum aerophilum |