Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
to 1.6 A resolution | Geobacillus kaustophilus |
Protein Variants | Comment | Organism |
---|---|---|
K267A | compared with wild-type, the affinity for the substrate analogue, 21 bp double-stranded DNA containing an apurinic/apyrimidinic-site analogue, is drastically decreased. The dissociation constant of the mutant is 0.38 microM | Geobacillus kaustophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | EndoIV contains two Fe2+ ions and one Zn2+ ion, crystallography data. Fe2+(1) is coordinated by Glu145, Asp179, His214, Glu259, and Fe2+(2) is coordinated by His69, His110 and Glu145 | Geobacillus kaustophilus | |
Zn2+ | EndoIV contains two Fe2+ ions and one Zn2+ ion, crystallography data. The Zn2+ ion is coordinated by His182, Asp227, His229 | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | Q5KX27 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
21 bp double-stranded DNA containing an apurinic/apyrimidinic-site analogue | - |
Geobacillus kaustophilus | ? | the affinity of EndoIV for the substrate analogue is very high and its dissociation constant is less than 0.01 microM. A C-terminal DNA-recognition loop at residues 265-269 that is only present in the long type enzymes contributes to its high affinity for apurinic/apyrimidinic sites | ? |
Synonyms | Comment | Organism |
---|---|---|
EndoIV | - |
Geobacillus kaustophilus |