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Literature summary for 4.2.99.18 extracted from

  • Kiyonari, S.; Tahara, S.; Shirai, T.; Iwai, S.; Ishino, S.; Ishino, Y.
    Biochemical properties and base excision repair complex formation of apurinic/apyrimidinic endonuclease from Pyrococcus furiosus (2009), Nucleic Acids Res., 37, 6439-6453.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Activating Compound

Activating Compound Comment Organism Structure
NaCl the highest AP endonuclease activity is observed at NaCl concentrations of 100-150 mM Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
E261A the mutant retains some activity Pyrococcus furiosus
H70A/H110A the mutant retains residual enzyme activity Pyrococcus furiosus
H70A/H110A/E261A the mutant completely loses the activity Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximally stimulated at 10 mM Pyrococcus furiosus
Mn2+ maximally stimulated at 10 mM Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U437
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Hitrap Q column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Pyrococcus furiosus fragments of DNA
-
 ?

Synonyms

Synonyms Comment Organism
AP endonuclease
-
 Pyrococcus furiosus
Ape
-
 Pyrococcus furiosus
apurinic/apyrimidinic endonuclease
-
 Pyrococcus furiosus
PF0258 the PF0258 protein exhibited AP endonuclease activity Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 in Tris-HCl buffer Pyrococcus furiosus

Expression

Organism Comment Expression
Pyrococcus furiosus the associated 3'-5' exonuclease activity but not the AP endonuclease activity of APE is stimulated by proliferating cell nuclear antigen additional information

General Information

General Information Comment Organism
physiological function AP endonuclease and proliferating cell nuclear antigen form a functional complex to generate a gap of several nucleotides for efficient DNA repair synthesis Pyrococcus furiosus