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Literature summary for 4.2.99.18 extracted from
- African swine fever virus protein pE296R is a DNA repair apurinic/apyrimidinic endonuclease required for virus growth in swine macrophages (2006), J. Virol., 80, 4847-4857.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pRSET-A vector for expression in Escherichia coli BL21DE3/pLys cells, the expression of pE296R protein is analyzed in ASFV-infected Vero cells | African swine fever virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
African swine fever virus | 5737 | - |
| nucleus | - |
African swine fever virus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the optimized buffer used for the endonuclease assay contains 5 mM MgCl2 | African swine fever virus |  |
| Zn2+ | the optimized buffer used for the exonuclease assay contains 5 mM ZnCl2 | African swine fever virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
determined by SDS-PAGE | African swine fever virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| African swine fever virus | - |
the swine macrophage is the host cell of the virus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-nitrilotriacetic acid agarose beads | African swine fever virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Vero cell | African swine fever virus | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| apurinic/apyrimidinic endonuclease | - |
African swine fever virus |
| pE296R | African swine fever virus, ASFV, protein pE296R, predicted to be a class II apurinic/apyrimidinic endonuclease | African swine fever virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
AP endonuclease assay and 3'-5' exonuclease assay | African swine fever virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
AP endonuclease assay and 3'-5' exonuclease assay | African swine fever virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | endonuclease activity | African swine fever virus |
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Release 2023.1 (January 2023)
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