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Literature summary for 4.2.99.18 extracted from

  • Mundle, S.T.; Fattal, M.H.; Melo, L.F.; Coriolan, J.D.; O'Regan, N.E.; Strauss, P.R.
    Novel role of tyrosine in catalysis by human AP endonuclease 1 (2004), DNA Repair, 3, 1447-1455.
    View publication on PubMed
Search for more literature for 4.2.99.18

Activating Compound

Activating Compound Comment Organism Structure
imidazole enhances activity of wild-type enzyme markedly, enhances Y171A somewhat and fails to enhance Y171F. When stoichiometric levels of tyrosine are included in the reaction with imidazole, wild-type enzyme as well as mutant enzymes are stimulated. Degree of stimulation of wild-type enzyme continues to exceed that of the mutants Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y128A the ratio of turnover number to Km-value for DNA containing an abasic site is 33fold lower than wild-type value at low salt concentration and 7.5fold lower than wild-type value at high salt concentrations Homo sapiens
Y171A enhancement by imidazole (in absence of tyrosine) is lower than that of wild-type enzyme. The ratio of turnover number to Km-value for DNA containing an abasic site is 50000fold lower than wild-type value at low salt concentration and 7500fold lower than wild-type value at high salt concentrations Homo sapiens
Y171F the ratio of turnover number to Km-value for DNA containing an abasic site is 25000fold lower than wild-type value at low salt concentration Homo sapiens
Y171H mutant enzyme is not enhanceed by imidazole (in absence of tyrosine). The ratio of turnover number to Km-value for DNA containing an abasic site is 50000fold lower than wild-type value at low salt concentration Homo sapiens
Y269A the ratio of turnover number to Km-value for DNA containing an abasic site is 12.5fold lower than wild-type value at low salt concentration and 21.4fold lower than wild-type value at high salt concentrations Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
100
-
 DNA containing an abasic site pH 7.5, wild-type enzyme, low salt concentration (82 mM NaCl) Homo sapiens
108
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y128A, high salt concentration (150 mM NaCl) Homo sapiens
110
-
 DNA containing an abasic site pH 7.5, wild-type enzyme, high salt concentration (150 mM NaCl) Homo sapiens
140
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y128A, low salt concentration (82 mM NaCl) Homo sapiens
165
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y269A, low salt concentration (82 mM NaCl) Homo sapiens
255
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171F, low salt concentration (82 mM NaCl) Homo sapiens
310
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171A, high salt concentration (150 mM NaCl) Homo sapiens
360
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171A, low salt concentration (82 mM NaCl) Homo sapiens
413
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y269A, high salt concentration (150 mM NaCl) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing an abasic site 45-mer oligomer Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
AP endonuclease I
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171A, high salt concentration (150 mM NaCl) Homo sapiens
0.0008
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171A, low salt concentration (82 mM NaCl) Homo sapiens
0.0009
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y171F, low salt concentration (82 mM NaCl) Homo sapiens
0.2
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y128A, high salt concentration (150 mM NaCl) Homo sapiens
0.3
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y269A, high salt concentration (150 mM NaCl) Homo sapiens
0.5
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y128A, low salt concentration (82 mM NaCl) Homo sapiens
1.3
-
 DNA containing an abasic site pH 7.5, mutant enzyme Y269A, low salt concentration (82 mM NaCl) Homo sapiens
1.6
-
 DNA containing an abasic site pH 7.5, wild-type enzyme, high salt concentration (150 mM NaCl) Homo sapiens
10
-
 DNA containing an abasic site pH 7.5, wild-type enzyme, low salt concentration (82 mM NaCl) Homo sapiens