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Literature summary for 4.2.99.18 extracted from

  • Purmal, A.A.; Rabow, L.E.; Lampman, G.W.; Cunningham, R.P.; Kow, Y.W.
    A common mechanism of action for the N-glycosylase activity of DNA N-glycosylase/AP lyases from E. coli and T4 (1996), Mutat. Res., 364, 193-207.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000015
-
 DNA containing thymine glycol pH 7.5 Escherichia coli
0.00000167
-
 DNA containing O-benzylhydroxylamine pH 7.5 Escherichia coli
0.00000284
-
 DNA containing O-methylhydroxylamine pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Escherichia coli
-
 fragments of DNA
-
 ?
DNA Tequatrovirus T4
-
 fragments of DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Tequatrovirus T4
-
-
-

Reaction

Reaction Comment Organism Reaction ID
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate mechanism Escherichia coli
a
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate mechanism Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Escherichia coli fragments of DNA
-
 ?
DNA
-
 Tequatrovirus T4 fragments of DNA
-
 ?
DNA duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates Escherichia coli fragments of DNA
-
 ?
DNA duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates Tequatrovirus T4 fragments of DNA
-
 ?
DNA recognizes urea, an oxidative ring fragmentation product of thymine Escherichia coli fragments of DNA
-
 ?
DNA containing O-benzylhydroxylamine
-
 Escherichia coli ?
-
 ?
DNA containing O-methylhydroxylamine
-
 Escherichia coli ?
-
 ?
DNA containing thymine glycol
-
 Escherichia coli ?
-
 ?