KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000015 | - |
DNA containing thymine glycol | pH 7.5 | Escherichia coli | |
0.00000167 | - |
DNA containing O-benzylhydroxylamine | pH 7.5 | Escherichia coli | |
0.00000284 | - |
DNA containing O-methylhydroxylamine | pH 7.5 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Escherichia coli | - |
fragments of DNA | - |
? | |
DNA | Tequatrovirus T4 | - |
fragments of DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate | mechanism | Escherichia coli | |
the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate | mechanism | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | - |
Escherichia coli | fragments of DNA | - |
? | |
DNA | - |
Tequatrovirus T4 | fragments of DNA | - |
? | |
DNA | duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates | Escherichia coli | fragments of DNA | - |
? | |
DNA | duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates | Tequatrovirus T4 | fragments of DNA | - |
? | |
DNA | recognizes urea, an oxidative ring fragmentation product of thymine | Escherichia coli | fragments of DNA | - |
? | |
DNA containing O-benzylhydroxylamine | - |
Escherichia coli | ? | - |
? | |
DNA containing O-methylhydroxylamine | - |
Escherichia coli | ? | - |
? | |
DNA containing thymine glycol | - |
Escherichia coli | ? | - |
? |