Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D283A | altered kinetic values | Homo sapiens |
D283A/D308A | altered kinetic values compared to wild-type enzyme | Homo sapiens |
D308A | altered kinetic values compared to wild-type enzyme | Homo sapiens |
H309N | altered kinetic values compared to wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
oligomer with G/U pair | D308A mutant, pH 7.5 | Homo sapiens | |
0.0001 | - |
oligomer with G/U pair | wild-type, pH 7.5 | Homo sapiens | |
0.00012 | - |
oligomer with G/U pair | D283A mutant, pH 7.5 | Homo sapiens | |
0.00024 | - |
oligomer with G/U pair | D283/D308A mutant, pH 7.5 | Homo sapiens | |
0.0013 | - |
oligomer with G/U pair | H309N mutant, pH 7.5 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Homo sapiens | - |
fragments of DNA | - |
? | |
additional information | Homo sapiens | key enzyme in repair of oxidatively damaged DNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | - |
Homo sapiens | fragments of DNA | - |
? | |
additional information | enzyme has no biologically significant 3'-5'-exonuclease activity, the activity only manifests at enzyme concentrations elevated by 6-7 orders magnitude, activity does not show a preference to mismatched compared to matched DNA structures as well as to nicked or gapped DNA substrates | Homo sapiens | ? | - |
? | |
additional information | key enzyme in repair of oxidatively damaged DNA | Homo sapiens | ? | - |
? | |
oligomer with G/U pair | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
oligomer with G/U pair | H309N mutant, pH 7.5 | Homo sapiens | |
0.02 | - |
oligomer with G/U pair | D283/D308A mutant, pH 7.5 | Homo sapiens | |
0.3 | - |
oligomer with G/U pair | D283A mutant, pH 7.5 | Homo sapiens | |
1.2 | - |
oligomer with G/U pair | D308A mutant, pH 7.5 | Homo sapiens | |
10 | - |
oligomer with G/U pair | wild-type, pH 7.5 | Homo sapiens |