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Literature summary for 4.2.99.18 extracted from
- Human placental apurinic/apyrimidinic endonuclease. Its isolation and characterization (1982), J. Biol. Chem., 257, 13455-13458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Homo sapiens |  |
| N-ethylmaleimide | - |
Homo sapiens | |
| NaCl | 50% inhibition by 50 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
DNA containing apurinic sites | - |
Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | optimal concentration: 3 mM | Homo sapiens | |
| Mn2+ | 1 mM | Homo sapiens | |
| Mn2+ | partial stimulation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Homo sapiens |
| 37000 | - |
1 * 37000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Homo sapiens | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 10 months, 50% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Homo sapiens | fragments of DNA | - |
? | |
| DNA | specific for DNA containing either apurinic or apyrimidinic sites | Homo sapiens | fragments of DNA | - |
? | |
| DNA containing apurinic sites | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.3 | pH 7.5: 50% of maximal activity, pH 8.3: activity maximum | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
NaCl | - |
Homo sapiens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | pH range 6-9 | - |
7.4 |
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Release 2023.1 (January 2023)
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