KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01403 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,6E)-farnesyl diphosphate | Nicotiana tabacum | formation of seven bisabolyl-derived sesquiterpenes through the intermediacy of a common (7R)-beta-bisabolyl cation, (+)-2-epiprezizaene (44%) is the major product, (-)-alpha-cedrene (21.5%), (R)-(-)-beta-curcumene (15.5%), alpha-acoradiene (3.9%), 4-epi-alpha-acoradiene (1.3%), and equal amounts of alpha-bisabolol (1.8%) and epi-alpha-bisabolol (1.8%). The enzyme may be able to exploit the low natural levels (3-14%) of (2Z,6E)-farnesyl diphosphate generated by farnesyl diphosphate synthases to produce a different array of sesquiterpenes in vivo. If the concentration of (2E,2E)-farnesyl diphosphate is depleted by its diversion into biosynthesis of phytosterols, farnesylated proteins, or diterpenes, the enzyme would be capable of utilizing (2Z,6E)-farnesyl diphosphate in its place | (-)-beta-curcumene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
recombinant enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,6E)-farnesyl diphosphate | formation of seven bisabolyl-derived sesquiterpenes through the intermediacy of a common (7R)-beta-bisabolyl cation, (+)-2-epiprezizaene (44%) is the major product, (-)-alpha-cedrene (21.5%), (R)-(-)-beta-curcumene (15.5%), alpha-acoradiene (3.9%), 4-epi-alpha-acoradiene (1.3%), and equal amounts of alpha-bisabolol (1.8%) and epi-alpha-bisabolol (1.8%). The enzyme may be able to exploit the low natural levels (3-14%) of (2Z,6E)-farnesyl diphosphate generated by farnesyl diphosphate synthases to produce a different array of sesquiterpenes in vivo. If the concentration of (2E,2E)-farnesyl diphosphate is depleted by its diversion into biosynthesis of phytosterols, farnesylated proteins, or diterpenes, the enzyme would be capable of utilizing (2Z,6E)-farnesyl diphosphate in its place | Nicotiana tabacum | (-)-beta-curcumene + diphosphate | - |
? | |
(2Z,6E)-farnesyl diphosphate | formation of seven bisabolyl-derived sesquiterpenes through the intermediacy of a common (7R)-beta-bisabolyl cation, (+)-2-epiprezizaene (44%) is the major product, (-)-alpha-cedrene (21.5%), (R)-(-)-beta-curcumene (15.5%), alpha-acoradiene (3.9%), 4-epi-alpha-acoradiene (1.3%), and equal amounts of alpha-bisabolol (1.8%) and epi-alpha-bisabolol (1.8%) | Nicotiana tabacum | (-)-beta-curcumene + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.77 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C | Nicotiana tabacum |