Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH not specified in the publication, presence of Mg2+ | Streptomyces clavuligerus | |
0.0027 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH not specified in the publication | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | B5GW45 | - |
- |
Streptomyces clavuligerus ATCC 27064 | B5GW45 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O = (+)-T-muurolol + diphosphate | cyclization mechanism involves an intermediate nerolidyl diphosphate leading to a helminthogermacradienyl cation. After a 1,3-hydride shift of the original H-1si of FPP, cyclization and deprotonation result in product (+)-T-muurolol | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces clavuligerus | (+)-T-muurolol + diphosphate | (+)-T-muurolol i.e. (1R,4R,4aS,8aR)-1,6-dimethyl-4-(propan-2-yl)-1,2,3,4,4a,7,8,8a-octahydronaphthalen-1-ol | ? | |
(2E,6E)-farnesyl diphosphate + H2O | cyclization mechanism a 1,3-hydride shift of the original H-1si of FPP | Streptomyces clavuligerus | (+)-T-muurolol + diphosphate | more than 95% of product | ? | |
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces clavuligerus ATCC 27064 | (+)-T-muurolol + diphosphate | (+)-T-muurolol i.e. (1R,4R,4aS,8aR)-1,6-dimethyl-4-(propan-2-yl)-1,2,3,4,4a,7,8,8a-octahydronaphthalen-1-ol | ? | |
(2E,6E)-farnesyl diphosphate + H2O | cyclization mechanism a 1,3-hydride shift of the original H-1si of FPP | Streptomyces clavuligerus ATCC 27064 | (+)-T-muurolol + diphosphate | more than 95% of product | ? |
Synonyms | Comment | Organism |
---|---|---|
SCLAV_p0068 | - |
Streptomyces clavuligerus |
SSCG_03688 | - |
Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00163 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH not specified in the publication, presence of Mg2+ | Streptomyces clavuligerus | |
0.00163 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH not specified in the publication | Streptomyces clavuligerus |