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Literature summary for 4.2.3.97 extracted from

  • Hu, Y.; Chou, W.K.; Hopson, R.; Cane, D.E.
    Genome mining in Streptomyces clavuligerus: expression and biochemical characterization of two new cryptic sesquiterpene synthases (2011), Chem. Biol., 18, 32-37.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.97

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication, presence of Mg2+ Streptomyces clavuligerus
0.0014
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus B5GS26
-
-
Streptomyces clavuligerus ATCC 27064 B5GS26
-
-

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = (-)-delta-cadinene + diphosphate cyclization mechanism involves an intermediate nerolidyl diphosphate leading to a helminthogermacradienyl cation. Following a 1,3-hydride shift of the original H-1si of FPP, cyclization and deprotonation will give (-)-delta-cadinene Streptomyces clavuligerus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Streptomyces clavuligerus (-)-delta-cadinene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate cyclization mechanism involves the intermediacy of nerolidyl diphosphate leading to a helminthogermacradienyl cation. Following a 1,3-hydride shift of the original H-1si of farnesyl diphosphate, cyclization and deprotonation will give (-)-delta-cadinene Streptomyces clavuligerus (-)-delta-cadinene + diphosphate more than 95% of product ?
(2E,6E)-farnesyl diphosphate
-
 Streptomyces clavuligerus ATCC 27064 (-)-delta-cadinene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate cyclization mechanism involves the intermediacy of nerolidyl diphosphate leading to a helminthogermacradienyl cation. Following a 1,3-hydride shift of the original H-1si of farnesyl diphosphate, cyclization and deprotonation will give (-)-delta-cadinene Streptomyces clavuligerus ATCC 27064 (-)-delta-cadinene + diphosphate more than 95% of product ?

Synonyms

Synonyms Comment Organism
SCreLAV_p0328
-
 Streptomyces clavuligerus
sscg_02150
-
 Streptomyces clavuligerus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00114
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication, presence of Mg2+ Streptomyces clavuligerus
0.00114
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication Streptomyces clavuligerus