Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00106 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Streptomyces avermitilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | - |
- |
- |
Streptomyces avermitilis SUKA17 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces avermitilis | avermitilol + diphosphate | avermitilol is the major product (85%), accompanied by germacrene A (1%), germacrene B (5%) and viridiflorol (3%), as well as several unidentified minor sesquiterpene products | ? | |
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces avermitilis SUKA17 | avermitilol + diphosphate | avermitilol is the major product (85%), accompanied by germacrene A (1%), germacrene B (5%) and viridiflorol (3%), as well as several unidentified minor sesquiterpene products | ? |
Synonyms | Comment | Organism |
---|---|---|
SAV 76 | - |
Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Streptomyces avermitilis |