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Literature summary for 4.2.3.95 extracted from

  • Lopez-Gallego, F.; Agger, S.A.; Abate-Pella, D.; Distefano, M.D.; Schmidt-Dannert, C.
    Sesquiterpene synthases Cop4 and Cop6 from Coprinus cinereus: catalytic promiscuity and cyclization of farnesyl pyrophosphate geometric isomers (2010), ChemBioChem, 11, 1093-1106.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.95

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Coprinopsis cinerea

Protein Variants

Protein Variants Comment Organism
N224D the mutant makes a small amount of (6S)-beta-bisabolene (18%) from (2Z,6E)-farnesyl diphosphate, the mutation does not affect the product profile of Cop6 with its normal substrate (2E,6E)-farnesyl diphosphate Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH not specified in the publication Coprinopsis cinerea
0.0076
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH 8.0 Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate conversion of (2E,6E)-farnesyl diphosphate proceeds via (3R)-nerolidyl diphosphate, a (6R)-beta-bisabolyl carbocation, and a (7R)-cuprenyl cation Coprinopsis cinerea (-)-alpha-cuprenene + diphosphate 98.2% yield ?
(2E,6E)-farnesyl diphosphate conversion of (2E,6E)-farnesyl diphosphate proceeds via a (6R)-beta-bisabolyl carbocation Coprinopsis cinerea (-)-alpha-cuprenene + diphosphate 98.2% yield ?
additional information cyclization of (2Z,6E)-farnesyl diphosphate by Cop6 proceeds through opposite enantiomers of a beta-bisabolyl cation intermediate and results in alpha-acoradiene + amorpha-4,11-diene Coprinopsis cinerea ?
-
 ?

Synonyms

Synonyms Comment Organism
(-)-alpha-cuprenene synthase
-
 Coprinopsis cinerea
Cop6
-
 Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH not specified in the publication Coprinopsis cinerea
0.67
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH 8.0 Coprinopsis cinerea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
88
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH not specified in the publication Coprinopsis cinerea
88
-
 (2E,6E)-farnesyl diphosphate at 25°C, pH 8.0 Coprinopsis cinerea