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Literature summary for 4.2.3.93 extracted from

  • Kumeta, Y.; Ito, M.
    Characterization of delta-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood (2010), Plant Physiol., 154, 1998-2007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE) Codon plus-RIL cells Aquilaria crassna

Protein Variants

Protein Variants Comment Organism
Y110S/V241E/P296S/H337Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Aquilaria crassna
Y110S/W144R/V241E/P296S/H337Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Aquilaria crassna

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000685
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna
0.00271
-
(2E,6E)-farnesyl diphosphate wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C Aquilaria crassna
0.00429
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna

Organism

Organism UniProt Comment Textmining
Aquilaria crassna
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Aquilaria crassna

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aquilaria crassna
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Aquilaria crassna delta-guaiene + diphosphate delta-guaiene is the major product (61% yield) ?

Synonyms

Synonyms Comment Organism
TPS
-
Aquilaria crassna

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000586
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna
0.00125
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna
0.00499
-
(2E,6E)-farnesyl diphosphate wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C Aquilaria crassna

Expression

Organism Comment Expression
Aquilaria crassna the production of delta-guaiene is induced by treatment with 300 mM methyl jasmonate reaching its maximum after 12 h up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.137
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna
0.182
-
(2E,6E)-farnesyl diphosphate mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C Aquilaria crassna
1.836
-
(2E,6E)-farnesyl diphosphate wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C Aquilaria crassna