Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE) Codon plus-RIL cells | Aquilaria crassna |
Protein Variants | Comment | Organism |
---|---|---|
Y110S/V241E/P296S/H337Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Aquilaria crassna |
Y110S/W144R/V241E/P296S/H337Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Aquilaria crassna |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000685 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
0.00271 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna | |
0.00429 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquilaria crassna | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Aquilaria crassna |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Aquilaria crassna | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | delta-guaiene + diphosphate | delta-guaiene is the major product (61% yield) | ? |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Aquilaria crassna |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000586 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
0.00125 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
0.00499 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna |
Organism | Comment | Expression |
---|---|---|
Aquilaria crassna | the production of delta-guaiene is induced by treatment with 300 mM methyl jasmonate reaching its maximum after 12 h | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
0.182 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
1.836 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna |