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Literature summary for 4.2.3.9 extracted from
- Templating effects in aristolochene synthase catalysis: elimination versus cyclisation (2011), Org. Biomol. Chem., 9, 6920-6923.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L108A | products are 6.8% aristolochene, 13.9% germacrene A, 26.9% (E)-beta-farnesene, 48.2%(E,E)-alpha-farnesene and 4.1% (E,Z)-alpha-farnesene | Penicillium roqueforti |
| L108F | products are 74.1% aristolochene, 13.8% germacrene A, 12% valencene | Penicillium roqueforti |
| L108S | products are 9.4% aristolochene, 14.6% germacrene A, 21.5% (E)-beta-farnesene, 49.1% (E,E)-alpha-farnesene and 5.3% (E,Z)-alpha-farnesene | Penicillium roqueforti |
| L108V | products are 88.3% aristolochene, 8% germacrene A, 1% valencene, and 2.3% (E,Z)-alpha-farnesene | Penicillium roqueforti |
| T89A | products are 93.4% aristolochene, 4.4% germacrene A, 2.2% valencene | Penicillium roqueforti |
| T89F | products are 67.6% aristolochene, 27.2% germacrene A, 5.2% valencene | Penicillium roqueforti |
| V88A | products are 86.2% aristolochene, 11.6% germacrene A, 2.2% valencene | Penicillium roqueforti |
| V88F | products are 18.4% aristolochene, 57.8% germacrene A, 23.8% valencene | Penicillium roqueforti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00053 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti |  |
| 0.00064 | - |
(2E,6E)-farnesyl diphosphate | mutant V88A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0007 | - |
(2E,6E)-farnesyl diphosphate | mutant V88F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.00175 | - |
(2E,6E)-farnesyl diphosphate | mutant T89A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.005 | - |
(2E,6E)-farnesyl diphosphate | mutant T89F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.7 | - |
(2E,6E)-farnesyl diphosphate | mutant L108A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.99 | - |
(2E,6E)-farnesyl diphosphate | mutant L108S, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 1.76 | - |
(2E,6E)-farnesyl diphosphate | mutant L108F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 2.03 | - |
(2E,6E)-farnesyl diphosphate | mutant L108V, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium roqueforti | Q03471 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Penicillium roqueforti | aristolochene + diphosphate | products are 91.5% aristolochene, 7.5% germacrene A, 1% valencene | ? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
(2E,6E)-farnesyl diphosphate | mutant V88F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0009 | - |
(2E,6E)-farnesyl diphosphate | mutant L108A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0011 | - |
(2E,6E)-farnesyl diphosphate | mutant T89F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0025 | - |
(2E,6E)-farnesyl diphosphate | mutant V88A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0036 | - |
(2E,6E)-farnesyl diphosphate | mutant L108F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0057 | - |
(2E,6E)-farnesyl diphosphate | mutant L108V, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0066 | - |
(2E,6E)-farnesyl diphosphate | mutant T89A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0095 | - |
(2E,6E)-farnesyl diphosphate | mutant L108S, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.084 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
(2E,6E)-farnesyl diphosphate | mutant V88F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0013 | - |
(2E,6E)-farnesyl diphosphate | mutant L108A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0021 | - |
(2E,6E)-farnesyl diphosphate | mutant L108F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0022 | - |
(2E,6E)-farnesyl diphosphate | mutant T89F, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0028 | - |
(2E,6E)-farnesyl diphosphate | mutant L108V, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0038 | - |
(2E,6E)-farnesyl diphosphate | mutant T89A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0039 | - |
(2E,6E)-farnesyl diphosphate | mutant V88A, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.0096 | - |
(2E,6E)-farnesyl diphosphate | mutant L108S, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
| 0.158 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Penicillium roqueforti | |
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