Any feedback?
Literature summary for 4.2.3.9 extracted from
- Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene (2002), J. Am. Chem. Soc., 124, 11636-11641.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme Y92F, expression in Escherichia coli | Penicillium roqueforti |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y92F | the mutant enzyme is approximately 0.1% as active as the nonmutated recombinant enzyme, the mutant releases significant amounts of germacrene A and also produces various amounts of a further five hydrocarbons of molecular weight 204, valencene, beta-(E)-farnesene, alpha-selinene, beta-selinene and selina-4,11-diene. The CD spectrum of the mutant enzyme is very similar to that of the wild-type enzyme | Penicillium roqueforti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
trans,trans-farnesyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Penicillium roqueforti |  |
| 0.1887 | - |
trans,trans-farnesyl diphosphate | pH 7.5, 30°C, mutant enzyme Y92F | Penicillium roqueforti | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium roqueforti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant enzymes | Penicillium roqueforti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans,trans-farnesyl diphosphate | - |
Penicillium roqueforti | aristolochene + diphosphate | 7.5% of the total amount of products are released from wild-type aristolochene synthase. The mutant enzyme Y92F releases significant amounts of germacrene A and also produces various amounts of a further five hydrocarbons of molecular weight 204, valencene, beta-(E)-farnesene, alpha-selinene, beta-selinene and selina-4,11-diene | ? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
trans,trans-farnesyl diphosphate | pH 7.5, 30°C, mutant enzyme Y92F | Penicillium roqueforti | |
| 0.03 | - |
trans,trans-farnesyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Penicillium roqueforti | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
