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Literature summary for 4.2.3.89 extracted from

  • Nakano, C.; Horinouchi, S.; Ohnishi, Y.
    Characterization of a novel sesquiterpene cyclase involved in (+)-caryolan-1-ol biosynthesis in Streptomyces griseus (2011), J. Biol. Chem., 286, 27980-27987.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.89

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000956
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C in the presence of 5 mM MgCl2 Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2 Streptomyces griseus
Mn2+ 38% activity (relative to the activity in the presence of Mg2+) is retained in the presence of 5 mM Mn2+ Streptomyces griseus
additional information no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+ Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-
Streptomyces griseus IFO 13350
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces griseus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces griseus (+)-beta-caryophyllene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces griseus IFO 13350 (+)-beta-caryophyllene + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Streptomyces griseus

Synonyms

Synonyms Comment Organism
GcoA
-
 Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 at pH 7.5 Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C in the presence of 5 mM MgCl2 Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 at 30°C Streptomyces griseus