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Literature summary for 4.2.3.87 extracted from
- Characterization of delta-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood (2010), Plant Physiol., 154, 1998-2007.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) Codon Plus-RIL cells | Aquilaria crassna |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| W144R/V241E/P296S/H337Y | inactive | Aquilaria crassna |
| Y110S/V241E/P296S/H337Y | the mutant produces 34.1% alpha-guaiene, 1.3% alpha-humulene and 64.6% delta-guaiene | Aquilaria crassna |
| Y110S/W144R/P296S/H337Y | inactive | Aquilaria crassna |
| Y110S/W144R/V241E/H337Y | inactive | Aquilaria crassna |
| Y110S/W144R/V241E/P296S | inactive | Aquilaria crassna |
| Y110S/W144R/V241E/P296S/H337Y | the mutant produces 31.1% alpha-guaiene, 1.5% alpha-humulene and 67.4% delta-guaiene | Aquilaria crassna |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquilaria crassna | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, gel filtration | Aquilaria crassna |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Aquilaria crassna | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | alpha-guaiene + diphosphate | 13% yield | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPS | - |
Aquilaria crassna |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Aquilaria crassna | the production of alpha-guaiene is induced by treatment with methyl jasmonate | up |
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