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Literature summary for 4.2.3.84 extracted from

  • Yu, F.; Harada, H.; Yamasaki, K.; Okamoto, S.; Hirase, S.; Tanaka, Y.; Misawa, N.; Utsumi, R.
    Isolation and functional characterization of a beta-eudesmol synthase, a new sesquiterpene synthase from Zingiber zerumbet Smith (2008), FEBS Lett., 582, 565-572.
    View publication on PubMed
Search for more literature for 4.2.3.84

Application

Application Comment Organism
synthesis introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l Zingiber zerumbet

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zingiber zerumbet

Protein Variants

Protein Variants Comment Organism
additional information introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l Zingiber zerumbet

Organism

Organism UniProt Comment Textmining
Zingiber zerumbet B1B1U4 var. Smith
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Source Tissue

Source Tissue Comment Organism Textmining
additional information no expression in leaf Zingiber zerumbet
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rhizome strong expression, transcript levels increase rapidly in summer, peak in early August and decline significantly in fall and winter Zingiber zerumbet
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stem poor expression Zingiber zerumbet
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
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 Zingiber zerumbet 10-epi-gamma-eudesmol + diphosphate products are 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene ?
(2E,6E)-farnesyl diphosphate + H2O
-
 Zingiber zerumbet Smith 10-epi-gamma-eudesmol + diphosphate products are 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene ?

Synonyms

Synonyms Comment Organism
ZSS2
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 Zingiber zerumbet