Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Santalum album |
expression in Escherichia coli | Santalum austrocaledonicum |
expression in Escherichia coli | Santalum spicatum |
heterologous expression of a His6-tagged protein in Escherichia coli | Santalum album |
heterologous expression of a His6-tagged protein in Escherichia coli | Santalum austrocaledonicum |
heterologous expression of a His6-tagged protein in Escherichia coli | Santalum spicatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum album | |
0.0014 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum austrocaledonicum | |
0.0014 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum spicatum | |
0.0014 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum album | |
0.0014 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum austrocaledonicum | |
0.0014 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum spicatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Santalum album | |
Mg2+ | required | Santalum austrocaledonicum | |
Mg2+ | required | Santalum spicatum | |
Mg2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum album | |
Mg2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum austrocaledonicum | |
Mg2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum spicatum | |
Mn2+ | incubation in presence of Mn2+ yields mainly alpha-exo-bergamotene | Santalum austrocaledonicum | |
Mn2+ | incubation in presence of Mn2+ yields mainly exo-alpha-bergamotene | Santalum spicatum | |
Mn2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum album | |
Mn2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum austrocaledonicum | |
Mn2+ | when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum spicatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65163 | - |
x * 65163, calculated from sequence | Santalum album |
65268 | - |
x * 65268, calculated from sequence | Santalum austrocaledonicum |
65407 | - |
x * 65407, calculated from sequence | Santalum spicatum |
66000 | - |
- |
Santalum album |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Santalum album | - |
(+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Santalum austrocaledonicum | - |
(+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Santalum spicatum | - |
(+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Santalum austrocaledonicum | - |
alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
(2E,6E)-farnesyl diphosphate | Santalum spicatum | - |
alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
(2E,6E)-farnesyl diphosphate | Santalum album | - |
alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
additional information | Santalum album | Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations | ? | - |
? | |
additional information | Santalum austrocaledonicum | Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene | ? | - |
? | |
additional information | Santalum spicatum | Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Santalum album | E3W202 | - |
- |
Santalum austrocaledonicum | E3W203 | - |
- |
Santalum spicatum | E3W204 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
xylem | - |
Santalum album | - |
xylem | - |
Santalum austrocaledonicum | - |
xylem | - |
Santalum spicatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Santalum album | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Santalum austrocaledonicum | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Santalum spicatum | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum album | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum austrocaledonicum | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene | Santalum spicatum | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | when incubated in the presence of 10 mM Mg2+ the enzyme converts (2Z,6Z)-farnesyl diphosphate into alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene and beta-santalene | Santalum album | (+)-alpha-santalene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Santalum austrocaledonicum | alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
(2E,6E)-farnesyl diphosphate | - |
Santalum spicatum | alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, alpha-exo-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
(2E,6E)-farnesyl diphosphate | - |
Santalum album | alpha-santalene + diphosphate | products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene | ? | |
additional information | Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations | Santalum album | ? | - |
? | |
additional information | Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene | Santalum austrocaledonicum | ? | - |
? | |
additional information | Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene | Santalum spicatum | ? | - |
? | |
additional information | Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene | Santalum album | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65163, calculated from sequence | Santalum album |
? | x * 65268, calculated from sequence | Santalum austrocaledonicum |
? | x * 65407, calculated from sequence | Santalum spicatum |
? | x * 66000, SDS-PAGE of recombinant His-tagged protein | Santalum album |
Synonyms | Comment | Organism |
---|---|---|
SaSSy | - |
Santalum album |
SspiSy | - |
Santalum spicatum |
SSy | - |
Santalum album |
SSy | - |
Santalum austrocaledonicum |
SSy | - |
Santalum spicatum |
SuSSy | - |
Santalum austrocaledonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Santalum album |
25 | - |
assay at | Santalum austrocaledonicum |
25 | - |
assay at | Santalum spicatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum album | |
0.34 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum album | |
0.91 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum austrocaledonicum | |
0.91 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum austrocaledonicum | |
2.6 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum spicatum | |
2.6 | - |
(2E,6E)-farnesyl diphosphate | 30°C, pH 8.0 | Santalum spicatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Santalum album |
7.4 | - |
assay at | Santalum austrocaledonicum |
7.4 | - |
assay at | Santalum spicatum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
242.9 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum album | |
650 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum austrocaledonicum | |
1857 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 25°C | Santalum spicatum |