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Literature summary for 4.2.3.8 extracted from
- High level expression of Ricinus communis casbene synthase in Escherichia coli and characterization of the recombinant enzyme (1996), Arch. Biochem. Biophys., 336, 283-289.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned, expressed and overproduced in Escherichia coli | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00176 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Ricinus communis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| proplastid | - |
Ricinus communis | 9537 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
1 * 59000, SDS-PAGE | Ricinus communis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | Ricinus communis | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | casbene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0026 | - |
recombinant enzyme | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | - |
Ricinus communis | casbene + diphosphate | - |
? | |
| geranylgeranyl diphosphate | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | Ricinus communis | casbene + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59000, SDS-PAGE | Ricinus communis |
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