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Literature summary for 4.2.3.77 extracted from

  • Picaud, S.; Olsson, M.E.; Brodelius, P.E.
    Improved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli (2007), Protein Expr. Purif., 51, 71-79.
    View publication on PubMed

Application

Application Comment Organism
synthesis improved conditions for expression of Zingiber officinale (+)-germacrene synthase in Escherichia coli. Comparison of bacterial strains; BL21 (DE3), BL21 (DE3) Tuner BL21(DE3) pLysS and BL21 (DE3) pLysS Tuner using different inducing agents. The change between BL21 (DE3) cells and BL21 (DE3) Tuner, induced with IPTG, leads to a twofold increase in enzyme activity in the soluble fraction while a reduction in activity is observed when using the pLysS strains. The same doubling of activity is observed for germacrene synthase when the commonly used BL.21 (DE3) is induced with The Inducer. Addition of 2.5 mM glycine betaine and 660 mM sorbitol to the bacterial growth media results in reduction of growth rate and biomass yield but under these conditions the best overall protein production is obtained Zingiber officinale

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zingiber officinale

Organism

Organism UniProt Comment Textmining
Zingiber officinale Q0VHD6
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